The Caspase Pathway as a Possible Therapeutic Target in Experimental Pemphigus

Abstract

Apoptosis plays a roles in phemphigus IgG-dependent acantholysis; theoretically, the blockade of the caspase pathway could prevent the blistering that is caused by pemphigus autoantibodies. Using this strategy, we attempted to block the pathogenic effect ofpemphigus IgG in Balb/c mice by using the caspase inhibitor Ac-DEVD-CMK. This inhibitor was administrated before theinjection of pemphigus IgG into neonatal mice. The main resultsof the present investigation are as follows: pemphigusIgG induces intraepidermal blisters in Balb/c neonatal mice; keratinocytes around the blister and acantholytic cells undergoapoptosis; the caspases inhibitor Ac-DEVD-CMK prevents apoptosis; the inhibition of the caspase pathway prevents blisterformation. In conclusion, inhibition of the caspase pathway may be a promising therapeutic tool that can help in the treatment ofpemphigus flare ups.