Please use this identifier to cite or link to this item: http://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/2506
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dc.contributor46461es_ES
dc.contributor.otherhttps://orcid.org/0000-0002-1995-1696-
dc.coverage.spatialGlobales_ES
dc.creatorLazalde Ramos, Blanca Patricia-
dc.creatorLares Asseff, Ismael-
dc.creatorVillanueva Fierro, Ignacio-
dc.creatorSosa Macías, Martha-
dc.creatorYahuaca Mendoza, Patricia-
dc.creatorZamora Perez, Ana-
dc.date.accessioned2021-05-25T19:08:41Z-
dc.date.available2021-05-25T19:08:41Z-
dc.date.issued2010-09-
dc.identifierinfo:eu-repo/semantics/publishedVersiones_ES
dc.identifier.issn1945-239Xes_ES
dc.identifier.urihttp://ricaxcan.uaz.edu.mx/jspui/handle/20.500.11845/2506-
dc.identifier.urihttps://doi.org/10.48779/55ar-t208-
dc.description.abstractThe aim of the present study was to modify and validate a high-performance liquid chromatographic (HPLC) method for determining 2,3 and 2,5 dihydroxybenzoic acid (2,3-DHBA and 2,5-DHBA) from salicylic acid in human plasma. The mobile phase was a mixture of sodium acetate/citrate (pH 2.5) 30 mM-methanol (93:7, v/v). The injection volume was 10 µL. Retention time for 2,5- DHBA, and 2,3-DHBA was 4.5 ± 0.10 and 5.8 ± 0.15 min, respectively. The detection and quantification limits were 10 and 40 nM for 2,3-DHBA and 8 and 20 nM for 2,5-DHBA. Linearity was evaluated in the range of 40–1600 nM for both metabolites. Inter-and intra-analysis variation coefficient was below 10%. Good recoveries of more than 99% were obtained for both metabolites using this method.es_ES
dc.language.isoenges_ES
dc.publisherOxford Academices_ES
dc.relationhttps://doi.org/10.1093/chromsci/48.8.675es_ES
dc.relation.urigeneralPublices_ES
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Estados Unidos de América*
dc.rightsAtribución-NoComercial-CompartirIgual 3.0 Estados Unidos de América*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.sourceJournal of Chromatographic Science, Vol. 48, Issue 8. p. 675–679,es_ES
dc.subject.classificationBIOLOGIA Y QUIMICA [2]es_ES
dc.subject.otherHPLC Methodes_ES
dc.subject.otherOxidative Stresses_ES
dc.subject.otherSalicilate Hydroxylationes_ES
dc.subject.otherHuman Plasmaes_ES
dc.titleHPLC Method for Quantification of Oxidative Stress by Salicilate Hydroxylation in Human Plasmaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
Appears in Collections:*Documentos Académicos*-- M. en Ciencias y Tecnología Química

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