Oxidative Stress Modulation by Rosmarinus offi cinalis in CCl4-induced Liver Cirrhosis

Abstract

Rosmarinus offi cinalis (Lamiaceae) possesses antioxidant activity and hepatoprotective effects, and so may provide a possible therapeutic alternative for chronic liver disease. The effect produced by a methanolic extract of Rosmarinus offi cinalis on CCl4-induced liver cirrhosis in rats was investigated using both prevention and reversion models. Over the course of the development of cirrhosis, the increased enzymatic activities of gammaglutamyl transpeptidase and alanine aminotransferase, and the rise in bilirubin levels caused by CCl4 administration, were prevented by Rosmarinus offi cinalis co-administration. When the cirrhosis by oxidative stress was evaluated as an increase on liver lipoperoxidation, total lipid peroxides, nitric oxide in serum, and loss of erythrocyte plasma membrane stability, R. offi cinalis was shown to prevent such alterations. On cirrhotic animals treated with CCl4, histological studies showed massive necrosis, periportal inflammation and fibrosis which were modified by R. offi cinalis. These benefits on experimental cirrhosis suggest a potential therapeutic use for R. offi cinalis as an alternative for liver cirrhosis. Copyright © 2009 John Wiley & Sons, Ltd.