Utilización de la autorradiografía en la detección de antígenos predominantes de Trichinella spiralis en tres modelos experimentales

Abstract

In this study, the detection of dominant antigens of Trichinella spiralis in three experimenta modeis (rat, mouse and rabbit), was carried out by Western Blot autoradiography (WB). Healthy animals were considered as controls. Experimenta animals were infected with infective larvae of T. spiralis: Animals were bleed before inoculation and at the fourth week post infection. The tri-chinella soluble antigens (AST) were obtained from muscles of experimentally infected rats. The AST were separated by reducing electrophoresis. Proteins in not stained gels were transferred to nitrocellulose paper sheets (NC), antibodies linked to NC were labeled with Staphylococcus aureus protein A SPA y (SPA) and the immune reactive bands were observed by autoradiography. By WB, T. spiralis infected animals recognized several antigenic componentes with a molecular weight ranging from 29 to 97 kDa, with dominant bands at 50, 47 and 45 kDa observed in 100% of studied sera. All controls were negative.